CESG Structural Genomics Platform Flowchart
After target selection, the process of structural determination begins with cloning sequencing. This sequencing information is sent to external databases like GenBank, PDB, and BMRB. The target for clone sequencing can either be as result of target selection or it can be from a cDNA library or clone library. After the target passes through cloning sequencing, it is processed by small-scale screening to test the likelihood it will pass through stages downstream. Following small-scale expression testing, the target is sent to large-scale production, followed by screening for suitability. If the protein passes this point, its structure is determined, refined, and later validated. At each of these checkpoints listed above, experimental information is deposited into Sesame (LIMS) program. Finally, the validated structure and associated data is used for futher functional studies and / or is published.
Targets progress through CESG's functional teams, in the following order, shown below, at left and at right, and can pass, or fail, at numerous points. Click on the team name to learn more about each team:
